Regulatory

Part:BBa_K4687004

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-07)


The promoter Ptac

We ligated the designed Ptac promoter sequence with CRISPR-MAD7 nuclease to construct an expression vector. We amplified the plasmid by transforming the expression vector into E. coli and verified whether it was correct by sequencing, and then transformed it into Corynebacterium glutamicum as a way to observe the expression effect of the CRISPR-MAD7 system. It was observed whether the Ptac promoter could enhance the expression of CRISPR-MAD7 system in Corynebacterium glutamicum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None